tlr4 ligand Search Results


lps  (Novus Biologicals)
94
Novus Biologicals lps
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Lps, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/LPS+from+E%2E+Coli%2C+TLR4+ligand/pmc08096850-178-50-51
Average 94 stars, based on 1 article reviews
lps - by Bioz Stars, 2026-11
94/100 stars
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ultrapure tlr4-specific lps escherichia coli  (Enzo Biochem)
90
Enzo Biochem ultrapure tlr4-specific lps escherichia coli
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Ultrapure Tlr4 Specific Lps Escherichia Coli, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/tlr4+ligand+lps/10__1074_slash_jbc__m110__170290-57-4-6
Average 90 stars, based on 1 article reviews
ultrapure tlr4-specific lps escherichia coli - by Bioz Stars, 2026-11
90/100 stars
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tlr4 ligand-lps  (ImmunoTools)
90
ImmunoTools tlr4 ligand-lps
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Tlr4 Ligand Lps, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/tlr4+ligand+lps/pmc07217920-250-36-47
Average 90 stars, based on 1 article reviews
tlr4 ligand-lps - by Bioz Stars, 2026-11
90/100 stars
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toll-like receptor [tlr]4 ligand lipopolysacharide [lps]  (List Biological Laboratories)
90
List Biological Laboratories toll-like receptor [tlr]4 ligand lipopolysacharide [lps]
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Toll Like Receptor [Tlr]4 Ligand Lipopolysacharide [Lps], supplied by List Biological Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/toll+like+receptor++tlr+4+ligand+lipopolysacharide++lps+/pm25240680-44-23-34
Average 90 stars, based on 1 article reviews
toll-like receptor [tlr]4 ligand lipopolysacharide [lps] - by Bioz Stars, 2026-11
90/100 stars
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lipid a (tlr4 ligand)  (Verlag GmbH)
90
Verlag GmbH lipid a (tlr4 ligand)
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Lipid A (Tlr4 Ligand), supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/lps++tlr4+ligand++from+e++coli+0+111+b4/pm20201020-335-0-16
Average 90 stars, based on 1 article reviews
lipid a (tlr4 ligand) - by Bioz Stars, 2026-11
90/100 stars
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2’3’-cgamp  (InvivoGen)
99
InvivoGen 2’3’-cgamp
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
2’3’ Cgamp, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/2%E2%80%993%E2%80%99-cGAMP/custom%40tlrl-nacga23-02%4010%2E1016%2Fj%2Ecelrep%2E2023%2E112306
Average 99 stars, based on 1 article reviews
2’3’-cgamp - by Bioz Stars, 2026-11
99/100 stars
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lipopolysaccharide/endotoxin, the tlr-4 ligand  (BioChem GmbH)
90
BioChem GmbH lipopolysaccharide/endotoxin, the tlr-4 ligand
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Lipopolysaccharide/Endotoxin, The Tlr 4 Ligand, supplied by BioChem GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/lipopolysaccharide+endotoxin++the+tlr+4+ligand/pm25541298-83-20-30
Average 90 stars, based on 1 article reviews
lipopolysaccharide/endotoxin, the tlr-4 ligand - by Bioz Stars, 2026-11
90/100 stars
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3d(6-acyl) phad  (Avanti Polar)
94
Avanti Polar 3d(6-acyl) phad
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
3d(6 Acyl) Phad, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/3D(6-acyl)+PHAD/custom%40699855p%4038968676
Average 94 stars, based on 1 article reviews
3d(6-acyl) phad - by Bioz Stars, 2026-11
94/100 stars
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human toll-like receptor 4 (tlr4) ligands  (Joint Research Center)
90
Joint Research Center human toll-like receptor 4 (tlr4) ligands
a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with <t>LPS</t> (1 µg/ml) <t>and</t> <t>ATP</t> (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).
Human Toll Like Receptor 4 (Tlr4) Ligands, supplied by Joint Research Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+ligand/human+toll+like+receptor+4++tlr4++ligands/pm21262374-9-71-114
Average 90 stars, based on 1 article reviews
human toll-like receptor 4 (tlr4) ligands - by Bioz Stars, 2026-11
90/100 stars
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lipopolysaccharide-lps from ecoli 0111:b4, tlr4 ligand  (AMS Biotechnology)
N/A
Lipopolysaccharide-LPS from Ecoli 0111:B4, TLR4 ligand
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lipopolysaccharide-lps from ecoli 0111:b4, tlr4 ligand  (Abeomics)
N/A
Lipopolysaccharide-LPS from Ecoli 0111:B4, TLR4 ligand
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Image Search Results


a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with LPS (1 µg/ml) and ATP (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).

Journal: Cell Discovery

Article Title: Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis

doi: 10.1038/s41421-021-00259-0

Figure Lengend Snippet: a Schematic representation of hPANX1 heptamer in light blue docked into the cryo-EM density map. The middle panel shows the top view of hPANX1 with glycosylation indicated. The right panel shows a magnified view of the N-linked glycans (including two N-Acetylglucosamine and eight Mannose). b Representative images of the localization of WT and N255Q hPANX1 in THP-1-derived macrophages (MPs). The images were collected using the super-resolution STED mode. OE is the abbreviation of overexpression in all the figures. Scale bar = 5 µm. c Western blot analysis of the NLRP3 and Pro-IL-1β in WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were treated with LPS (1 µg/ml) and ATP (3 mM) for 24 h. GAPDH was used as a loading control. d IL-1β release from mock, WT hPANX1 overexpressing and N255Q hPANX1 overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). e Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. f Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).

Article Snippet: To differentiate THP-1 cells into macrophages (MPs), 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, P1585–1MG) was added to the growth medium and maintained for 24 or 48 h. THP-1 derived MPs were stimulated for the indicated time with 30 μM R848, pHrodo E. coli bioparticles (Life Technologies, P35361), 1 μg/ml LPS (Novus Biologicals, NBP2-25295), R848 plus bioparticles, LPS plus 3 mM ATP (Sigma-Aldrich, A2383-5G), or primed with LPS and stimulated with 10 μM nigericin (MERCK Sigma-Aldrich, 481990).

Techniques: Cryo-EM Sample Prep, Glycoproteomics, Derivative Assay, Over Expression, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Comparison

a Representative images of the localization of WT and mutant hPANX1 (D379A and S424A) in MPs. The images were collected using the super-resolution STED mode. Scale bar = 5 µm. b Western blot analysis of the NLRP3, Pro-IL-1β in WT and mutant (D379A and S424A) hPANX1 overexpressing MPs. MPs were treated with LPS (1 µg/ml) and ATP (3 mM) for 24 h. GAPDH was used as a loading control. c IL-1β release from mock, WT overexpressing and mutant (D379A and S424A) overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, ** P < 0.01, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). d Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. e Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).

Journal: Cell Discovery

Article Title: Structure of the full-length human Pannexin1 channel and insights into its role in pyroptosis

doi: 10.1038/s41421-021-00259-0

Figure Lengend Snippet: a Representative images of the localization of WT and mutant hPANX1 (D379A and S424A) in MPs. The images were collected using the super-resolution STED mode. Scale bar = 5 µm. b Western blot analysis of the NLRP3, Pro-IL-1β in WT and mutant (D379A and S424A) hPANX1 overexpressing MPs. MPs were treated with LPS (1 µg/ml) and ATP (3 mM) for 24 h. GAPDH was used as a loading control. c IL-1β release from mock, WT overexpressing and mutant (D379A and S424A) overexpressing MPs. MPs were primed with LPS (1 µg/ml) overnight and stimulated with nigericin (10 µM) for 0.5, 1, or 2 h. IL-1β release was measured by ELISA. Error bars represent s.e.m. for n = 3. * P < 0.05, ** P < 0.01, NS not significant (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control). d Phase-contrast images of MPs stimulated by LPS and ATP. Images were acquired with an IncusyteS3 system on day 6. e Time course of live cell confluence of MPs. Image data were collected every 2 h and analyzed with an IncusyteS3 system. Error bars represent s.d. for n = 4. *** P < 0.001, **** P < 0.0001 (one-way ANOVA followed by Dunnett’s test, OE WT set as comparison control).

Article Snippet: To differentiate THP-1 cells into macrophages (MPs), 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, P1585–1MG) was added to the growth medium and maintained for 24 or 48 h. THP-1 derived MPs were stimulated for the indicated time with 30 μM R848, pHrodo E. coli bioparticles (Life Technologies, P35361), 1 μg/ml LPS (Novus Biologicals, NBP2-25295), R848 plus bioparticles, LPS plus 3 mM ATP (Sigma-Aldrich, A2383-5G), or primed with LPS and stimulated with 10 μM nigericin (MERCK Sigma-Aldrich, 481990).

Techniques: Mutagenesis, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Comparison